MicrobiologybioRxiv
Probing the fabric of the rare biosphere
Bibiana G. Crespo, Philip J. Wallhead, Ramiro Logares, Carlos Pedros-Alio
The paper investigates the rare biosphere using high-throughput sequencing and culturing, showing that isolation captures rare taxa and that increased sequencing depth gradually reveals more bacteria.
DOI 10.1371/journal.pone.0159195