Heterologous expression and purification of glutamate decarboxylase-1 from the model plant Arabidopsis thaliana - characterization of the enzyme's in vitro truncation by thiol endopeptidase activity
Brittany S. Menard, Kirsten H. Benidickson, Lee Marie Raytek, Wayne A. Snedden, William Charles Plaxton
This paper investigates proteolytic truncation of recombinant AtGAD1 during purification and its impact on enzyme activity.
Protein Expression and Purification · Sep 27, 2024Peer-reviewedBiochemistry