Purification of synchronized E. coli transcription elongation complexes by reversible immobilization on magnetic beads
S. L. Kelly, C. E. Szyjka, E. J. Strobel
This paper develops a method to purify synchronized E. coli transcription elongation complexes using a custom leader sequence, enabling high-purity, active, and synchronous TECs for RNA assays.
bioRxiv · Jan 10, 2022Biochemistry