Multiplexed bioluminescence microscopy via phasor analysis
Z. Yao, C. K. Brennan, L. Scipioni, H. Chen, K. Ng, M. Digman, J. A. Prescher
This paper introduces a method combining bioluminescence with phasor analysis to rapidly differentiate multiple luciferase reporters and resonance energy transfer processes in time-lapse imaging.
bioRxiv · Jun 18, 2021Bioengineering