L. Scipioni

Proximity Labeling Expansion Microscopy (PL-ExM) resolves structure of the interactome

PL-ExM combines proximity labeling and expansion microscopy to visualize 3D interactome structures and assess PL technique performance.

Building Fluorescence Lifetime Maps Photon-by-photon by Leveraging Spatial Correlations

This paper introduces a doubly nonparametric framework for FLIM that learns fluorescent species and absolute lifetime maps while addressing spatial correlations and low photon counts.

Fluorescence Lifetime: Beating the IRF and interpulse window

This paper introduces BNP-LA, a Bayesian nonparametric method for analyzing fluorescence lifetime data with arbitrary lifetimes, and demonstrates its effectiveness using synthetic and experimental data.

Epigenetic dysregulation from chromosomal transit in micronuclei

This study shows that chromosomal transit through micronuclei causes epigenetic dysregulation in cancer cells, leading to long-term chromatin accessibility abnormalities.

Multiplexed bioluminescence microscopy via phasor analysis

This paper introduces a method combining bioluminescence with phasor analysis to rapidly differentiate multiple luciferase reporters and resonance energy transfer processes in time-lapse imaging.

Nanoscale distribution of nuclear sites analyzed by superresolution STED-ICCS

This paper introduces STED-ICCS, a method combining superresolution microscopy with image cross-correlation spectroscopy to quantify nanoscale spatial distribution of functional nuclear sites in MCF10A cells.

Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response.

The paper develops a biophysical method to quantify chromatin compaction dynamics during DNA damage response using FLIM-FRET microscopy and identifies roles of ATM and RNF8 in chromatin reorganization.