Understanding boundary effects and confocal optics enables quantitative FRAP analysis in the confined geometries of animal, plant, and fungal cells
James L. Kingsley, Jeffrey P. Bibeau, S. Iman Mousavi, Cem Unsal, Zhilu Chen, Xinming Huang, Luis Vidali, Erkan Tüzel
The paper develops a 3D computational model of FRAP incorporating cell boundaries and confocal optics, validates it in plant cells, and shows how these factors affect FRAP recovery curve interpretation.
bioRxiv · Jun 15, 2016 · PreprintBiophysics