Catalytically Enhanced Cas9 through Directed Protein Evolution
Travis H. Hand, Mitchell O. Roth, Chardasia L. Smith, Emily A. Shiel, Kyle N. Klein, David M. Gilbert, Hong Li
This paper describes a directed protein evolution method to enhance the catalytic efficiency of CRISPR-Cas9 variants, showing up to 4-fold improvement in AceCas9 and 2-fold increase in HDR-based gene insertion for SpyCas9.
The CRISPR Journal · Apr 1, 2021Peer-reviewedSynthetic Biology