Hong Li

This paper reconstructs extinct glucokinase (GCK) and glucokinase regulatory protein (GKRP) to study the evolutionary origins of protein regulation in vertebrate glucose homeostasis.

This study determines the structural basis of how Ec78 retrons co-opt the PtuAB ATPase-nuclease system for anti-phage defense through cryo-EM and biochemical analysis.

This paper identifies a CRISPR-associated protein, Cad1, that converts ATP to ITP in a cOA-dependent manner through an allosteric mechanism.

This study investigates how pseudouridine affects ribosome susceptibility to aminoglycosides by comparing wild-type and pseudouridine-deficient ribosomes.

The paper develops a non-viral method using hybrid ssDNA repair templates to improve genome engineering in primary human cells, achieving high knock-in efficiency and cell yields for disease modeling and cell therapy.

This paper describes a directed protein evolution method to enhance the catalytic efficiency of CRISPR-Cas9 variants, showing up to 4-fold improvement in AceCas9 and 2-fold increase in HDR-based gene insertion for SpyCas9.