Targeted Deletion of Vitamin D receptor Gene in Mammalian Cells by CRISPR/Cas9 Systems
Tao zhang, Ling Wang, Kun Xu, Chonghua Ren, Zhongtian Liu, Zhiying Zhang
This study demonstrates that CRISPR/Cas9 can delete large genomic segments in human and mouse cells by using two sgRNAs targeting conserved regions of the VDR gene.
bioRxiv · Feb 18, 2016 · PreprintGenomics