DNA isolation protocol effects on nuclear DNA analysis by microarrays, droplet digital PCR, and whole genome sequencing, and on mitochondrial DNA copy number estimation
Elizabeth P. Nacheva, Katya Mokretar, Aynur Soenmez, Alan Michael Pittman, Colin D. Grace, Roberto Valli, Ayesha Ejaz, Selina Vattathil, Emanuela Maserati, Henry Houlden, Jan‐Willem Taanman, Anthony H.V. Schapira, Christos Proukakis
The paper shows DNA isolation methods affect array CGH, ddPCR, and whole genome sequencing results in human brain tissue, with protocol-dependent biases in GC coverage and mtDNA copy number.
PLoS ONE · Jul 6, 2017Peer-reviewedGenomics