Mapping protein function with CRISPR/Cas9-mediated mutagenesis
Katherine F Donovan, Mudra Hegde, Meagan Sullender, Emma W Vaimberg, Cory M Johannessen, David E Root, John G Doench
The paper uses CRISPR/Cas9 mutagenesis to generate allelic variants of MEK1 and BRAF, identifying gain-of-function alleles in the MAPK signaling pathway.
bioRxiv · Sep 23, 2016Genomics