Choice of 16S ribosomal RNA primers impacts urinary microbiota profiling
V. Heidrich, L. T. Inoue, P. F. Asprino, F. Bettoni, A. C. H. Mariotti, D. A. Bastos, D. L. F. Jardim, M. A. Arap, A. A. Camargo
The paper evaluates the performance of different 16S rRNA hypervariable regions for urinary microbiota profiling and finds that V1V2 regions are more suitable for this purpose.
bioRxiv · Jan 24, 2022Microbiology