A CRISPRi-based genetic resource to study essential Staphylococcus aureus genes
P. Reed, M. Sorg, D. Alwardt, L. Serra, H. Veiga, S. Schaeper, M. G. Pinho
The paper develops a CRISPRi system for gene knockdown in S. aureus and constructs a library of 261 strains targeting 200 essential genes.
bioRxiv · Oct 31, 2022Microbiology