Safe and easy evaluation of tmRNA-SmpB-mediated trans-translation in ESKAPE pathogenic bacteria
M. Thepaut, R. Campos Da Silva, E. Renard, F. Barloy-Hubler, E. Ennifar, D. Boujard, R. Gillet
The paper develops a cell-free assay using GFP reassembly to evaluate trans-translation in ESKAPE bacteria for high-throughput screening and mechanistic studies.
bioRxiv · Dec 16, 2020Biochemistry