Cryo-electron tomography of large biological specimens vitrified by plunge freezing
F. J. B. Baeuerlein, M. Renner, D. El Chami, S. E. Lehnart, J. C. Pastor-Pareja, R. Fernandez-Busnadiego
This study demonstrates that plunge freezing can vitrify large biological specimens like mouse cardiomyocytes and Drosophila tissues, enabling efficient cryo-FIB/ET analysis of their molecular architecture.
bioRxiv · Apr 14, 2021 · PreprintCell Biology