Purification of recombinant α-Synuclein: a comparison of commonly used protocols
A. D. Stephens, D. Matak-Vinkovic, G. S. Kaminski Schierle
The paper compares four protocols for isolating recombinant α-synuclein from E. coli, finding that acid precipitation and periplasmic lysis yield the highest purity and monomer content.
bioRxiv · May 13, 2020Biophysics