A. D. Stephens

Nuclear blebs are associated with destabilized chromatin packing domains

The paper investigates how nuclear blebs alter chromatin packing domains, finding fragmentation of nanoscopic heterochromatin domains within blebs.

DNA density is a better indicator of a nuclear bleb than lamin B loss

The paper compares lamin B and DNA levels in nuclear blebs across cell types and perturbations, finding DNA concentration is more consistently reduced than lamin B levels.

CTCF is essential for proper mitotic spindle structure and anaphase segregation

This study shows that CTCF is crucial for correct mitotic spindle structure and anaphase segregation, leading to abnormal nuclear morphology in interphase.

Actin contraction controls nuclear blebbing and rupture independent of actin confinement

This study shows that actin contraction drives nuclear blebbing and rupture independently of actin confinement, with lamin B1 playing a key role in chromatin decompaction phenotypes.

Transcription regulates bleb formation and stability independent of nuclear rigidity

This study shows transcription affects nuclear bleb formation and stability independently of chromatin structure or nuclear rigidity.

Intracellular FUS protein accumulation leads to cytoskeletal, organelle and cellular homeostasis perturbations

This study investigates how mutant FUS protein accumulation affects cytoskeletal, organelle, and cellular homeostasis, linking it to cellular aging and potential senescence mechanisms.

Intracellular Aβ42 aggregation leads to cellular thermogenesis

This paper shows that Aβ42 aggregation in live cells increases intracellular temperature, which can be mitigated by aggregation inhibitors and is independent of mitochondrial damage.

The role of water mobility in protein misfolding

This study shows that water mobility, influenced by ions like NaCl and CsI, affects the aggregation rate of α-synuclein, a protein linked to Parkinson's disease.

HP1α is a chromatin crosslinker that controls nuclear and mitotic chromosome mechanics

This study shows HP1α crosslinks chromatin to maintain nuclear and mitotic chromosome mechanics, independent of histone methylation.

Correlating nuclear morphology and external force with combined atomic force microscopy and light sheet imaging separates roles of chromatin and lamin A/C in nuclear mechanics

The paper uses AFM-LS to correlate nuclear morphology and external force, revealing distinct roles of chromatin and lamin A/C in nuclear mechanics.

Fast purification of recombinant monomeric amyloid-β from E. coli and amyloid-β-mCherry aggregates from mammalian cells

This paper presents a rapid protocol for purifying monomeric recombinant Aβ from E. coli and fluorescent Aβ-mCherry from mammalian cells, avoiding aggregation and freeze-drying steps.

Purification of recombinant α-Synuclein: a comparison of commonly used protocols

The paper compares four protocols for isolating recombinant α-synuclein from E. coli, finding that acid precipitation and periplasmic lysis yield the highest purity and monomer content.

Short hydrogen bonds enhance non-aromatic protein-related fluorescence

The paper shows that short hydrogen bonds in L-glutamine enhance non-aromatic fluorescence through reduced non-radiative transitions, offering insights for bioactive material design.

Extent of N-terminus exposure by altered long-range interactions of monomeric alpha-synuclein determines its aggregation propensity

This study investigates how calcium-induced conformational changes in monomeric alpha-synuclein affect N-terminus exposure and aggregation propensity, identifying sequence-specific conformational differences as key factors.

Effects of altering histone post-translational modifications on mitotic chromosome structure and mechanics

This study investigates how histone post-translational modifications affect mitotic chromosome stiffness using force measurements and epigenetic drugs, finding that methylation, not acetylation, contributes to mitotic structure.

Multimodal interferometric imaging of nanoscale structure and macromolecular motion uncovers UV induced cellular paroxysm

This paper introduces a multimodal interferometric imaging platform to study nanoscale structure and macromolecular dynamics in live cells, revealing a new phenomenon called cellular paroxysm induced by UV light.

Physicochemical mechanotransduction alters nuclear shape and mechanics via heterochromatin formation

This study shows that extracellular cations alter nuclear shape and mechanics through heterochromatin formation, rescuing nuclear morphology in disease models.

Intrinsically aggregation-prone proteins form amyloid-like aggregates and contribute to tissue aging in C. elegans

This study shows that age-dependent protein aggregation in C. elegans forms amyloid-like structures and accelerates tissue functional decline, suggesting a role for protein aggregation in aging.

Chromatin histone modifications and rigidity affect nuclear morphology independent of lamins

The paper shows that chromatin histone modifications affect nuclear rigidity and morphology independently of lamins, inducing or suppressing nuclear blebs through changes in chromatin compaction.

Mechanics and buckling of biopolymeric shells and cell nuclei

This study uses simulations and experiments to show how biopolymeric shells and cell nuclei deform under tension, exhibiting different mechanical behaviors depending on force magnitude and temperature.