Cas9 is mostly orthogonal to human systems of DNA break sensing and repair
E. A. Maltseva, I. A. Vasil'eva, N. A. Moor, D. V. Kim, N. S. Dyrkheeva, M. M. Kutuzov, I. P. Vokhtantsev, L. M. Kulishova, D. O. Zharkov, O. I. Lavrik
This study shows that Cas9 is largely independent of human DNA break sensing and repair systems, as it does not significantly interact with key repair factors and shields breaks from detection.
bioRxiv · Sep 5, 2023Biochemistry