D. Prakoso

Leptospira transcriptome sequencing using long-read technology reveals unannotated transcripts and potential polyadenylation of mRNA molecules

This study uses long-read sequencing to analyze Leptospira transcriptomes, identifying unannotated transcripts and potential polyadenylation, with implications for virulence.

Leptospira enrichment culture followed by ONT Nanopore sequencing allows better detection of Leptospira presence and diversity in water and soil samples.

The paper compares methods for detecting Leptospira in environmental samples, finding that enrichment culture followed by PCR or sequencing improves detection of both pathogenic and nonpathogenic Leptospira.