Leptospira enrichment culture followed by ONT Nanopore sequencing allows better detection of Leptospira presence and diversity in water and soil samples.
M. Gorman, R. Xu, D. Prakoso, L. C. M. Salvador, S. Rajeev
The paper compares methods for detecting Leptospira in environmental samples, finding that enrichment culture followed by PCR or sequencing improves detection of both pathogenic and nonpathogenic Leptospira.
bioRxiv · Jun 16, 2022 · PreprintMicrobiology