A novel mechanistic framework for precise sequence replacement using reverse transcriptase and diverse CRISPR-Cas systems
Y. Bill Kim, Elizabeth B. Pierce, Michael Brown, Brenda A. Peterson, Derek Sanford, Justin M. Fear, David Nicholl, Ellyce San Pedro, Grace M. Reynolds, Joanne E. Hunt, David G. Schwark, Sathya Jali, Nathaniel D. Graham, Zoe Cesarz, Tracey A. Lincoln Chapman, , Aaron W. Hummel
This paper introduces REDRAW, a novel DNA editing method using reverse transcriptase and type V CRISPR-Cas systems, enabling broader sequence replacement beyond type II systems.
bioRxiv · Dec 13, 2022 · PreprintMolecular Biology