IVT generation of guideRNAs for Cas9-enrichment Nanopore Sequencing
Timothy Gilpatrick, Josh Zhiyong Wang, David Weiss, Alexis L. Norris, James R. Eshleman, Winston Timp
The paper describes a method using in vitro transcribed guideRNAs for targeted nanopore sequencing to study structural variations in pancreatic cancer cell lines.
bioRxiv · Feb 7, 2023 · PreprintMolecular Biology