Deciphering the History of ERK Activity from Fixed-Cell Immunofluorescence Measurements
Abhineet R. Ram, Michael Pargett, Yongin Choi, Devan A. Murphy, Carolyn Teragawa, Markhus Cabel, Nont Kosaisawe, Gerald Quon, John G. Albeck
The paper develops a framework to infer ERK signaling dynamics from fixed-cell immunostains by combining machine learning and differential equations, showing that protein levels reflect different activation timescales.
Nature Communications · May 21, 2025Peer-reviewedMolecular Biology