Orthogonal Transposons for Iterative Genome Engineering of Mammalian Cells.
Maggie Lee, Sowmya Rajendran, Divya Teja Vavilala, Lynn Webster, Indira Kottayil, Ferenc L. Boldog, Mario Pereira, Maya Wright, Surya Karunakaran, Molly Hunter, Varsha Sitaraman, Claes Gustafsson, Jeremy Minshull
This paper introduces a method using orthogonal transposons to iteratively modify the CHO genome for therapeutic development, achieving stable and predictable genetic modifications.
bioRxiv · Mar 27, 2026 · PreprintBioengineering