Structures of Cas9-Bound Double-Stranded DNA Mini-Circle Reveal Impacts of DNA Shape on Cas9 Target Interrogation
Kyu-Yeon Lee, Donggyun Kim, Yukang Liu, Hongjian Chang, Vadim Cherezov, Jiansen Jiang, Peter Zhifeng Qin
This study solves cryo-EM structures of SpyCas9 bound to minicircle DNA, revealing how DNA shape affects Cas9 target interrogation and cleavage.
bioRxiv · May 22, 2026 · PreprintBiophysics