Short hairpin RNAs artifactually impair cell growth and suppress clustered microRNA expression.
JT Powers, EL da Rocha, DS Pearson, P Missios, TY de Soysa, J Barragan, P Cahan, GQ Daley
This study shows that shRNAs impair cell growth and suppress microRNA expression in a sequence-independent manner, highlighting potential artifacts in cancer research.
bioRxiv · Dec 11, 2018 · PreprintCancer Biology