Shortcut barcoding and early pooling for scalable multiplex single-cell reduced-representation CpG methylation sequencing at single nucleotide resolution
Liyao Mai, Zebin Wen, Yulong Zhang, Yu Gao, Guanchuan Lin, Zhiwei Lian, Xiang Yang, Jingjing Zhou, Xianwei Lin, Chaochao Luo, Wanwan Peng, Caiming Chen, Jiajia Peng, Duolian Liu, Sadie L. Marjani, , Xinghua Pan
The paper introduces msRRBS, a scalable single-cell methylation sequencing method, achieving high read mapping rates and cost efficiency for analyzing methylation patterns in multiple cell lines and cancer models.
Nucleic Acids Research · Oct 4, 2023Peer-reviewedGenomics