Accumulation of an unprecedented 5'-deoxyadenos-4'-yl radical unmasks the kinetics of the radical-mediated C-C bond formation step in MoaA catalysis
H. Pang, E. A. Lilla, P. Zhang, D. Zhang, T. P. Shields, L. G. Scott, W. Yang, K. Yokoyama
This paper characterizes the kinetics of the radical-mediated C-C bond formation in MoaA, revealing a shunt pathway and determining the rate of the 3',8-cyclization step.
bioRxiv · Jan 16, 2020 · PreprintBiochemistry