Obtaining deeper insights into microbiome diversity using a simple method to block host and non-targets in amplicon sequencing
T. Mayer, A. Mari, J. Almario, M. Murillo-Roos, M. Abdullah, N. Dombrowski, S. Hacquard, E. M. Kemen, M. T. Agler
The paper introduces blocking oligos and an R package to reduce non-target DNA amplification in amplicon sequencing, improving microbial diversity analysis in plant systems.
bioRxiv · Oct 5, 2020Microbiology