Nonspecific Cleavages Arising from Reconstitution of Trypsin under Mildly Acidic Conditions
B. Niu, M. Martinelli, Y. Jiao, E. Meinke, M. Cao, J. Wang
This study shows that reconstitution and storage conditions of trypsins can significantly affect nonspecific cleavages in tryptic digestion, with mildly acidic conditions promoting up to 20% nonspecific activity.
bioRxiv · May 26, 2020Biochemistry