Identification of Transient Intermediates During Spliceosome Activation by Single Molecule Fluorescence Microscopy
X. Fu, H. Kaur, M. L. Rodgers, E. Montemayor, S. E. BUTCHER, A. Hoskins
This study uses single molecule fluorescence to identify a transient intermediate in yeast spliceosome activation involving Lsm2-8 and the NTC, revealing kinetic interactions not seen in cryo-EM structures.
bioRxiv · Apr 23, 2022Biochemistry