Direct RNA sequencing reveals structural differences between transcript isoforms
A. J. G. Aw, S. Lim, J. X. Wang, Y. Shen, P. Kaewsapsak, E. P. L. Kok, C. Li, B. H. Ng, L. A. Vardy, M. H. Tan, N. Nagarajan, Y. Wan
This study introduces PORE-cupine, a method combining structure probing and nanopore sequencing to detect RNA secondary structures, revealing isoform-specific structural differences and their impact on translation efficiency.
bioRxiv · Jun 11, 2020 · PreprintBiochemistry