E. R. Smithwick

Controllable multi-halogenation of a non-native substrate by SyrB2 iron halogenase

This paper demonstrates how SyrB2 iron halogenase can controllably halogenate non-native substrates to produce mono-, di-, and tri-chlorinated products by adjusting cofactor loading and electron transfer.

Equilibrium dialysis with HPLC detection to measure substrate binding affinity of a non-heme iron halogenase

The paper describes a method using equilibrium dialysis and HPLC to measure the substrate binding affinity (Kd) of the non-heme iron halogenase BesD for lysine.

Electrostatically regulated active site assembly governs reactivity in non-heme iron halogenases

This study investigates how anion and substrate binding cooperatively regulate reactivity in non-heme iron halogenases through electrostatic interactions in the active site.