A. Bhagi-Damodaran

Equilibrium dialysis with HPLC detection to measure substrate binding affinity of a non-heme iron halogenase

The paper describes a method using equilibrium dialysis and HPLC to measure the substrate binding affinity (Kd) of the non-heme iron halogenase BesD for lysine.

Oxygen affinities of DosT and DosS sensor kinases with implications for hypoxia adaptation in Mycobacterium tuberculosis

This study measures the oxygen binding affinities of DosT and DosS kinases in Mycobacterium tuberculosis, revealing stronger affinities than previously reported and linking them to hypoxia adaptation stages.

Gas tunnel engineering of prolyl hydroxylasereprograms hypoxia signaling in cells

This study reprograms hypoxia signaling by engineering PHD2 to enhance its catalytic efficiency, leading to reduced HIF-1 and hypoxia response transcripts under low oxygen conditions.

Understanding ATP binding to DosS catalytic domain with a short ATP-lid

This paper investigates ATP binding in the short ATP-lid of DosS CA domain, showing it binds ATP under physiological conditions and revealing conformational flexibility affected by zinc concentration.

Electrostatically regulated active site assembly governs reactivity in non-heme iron halogenases

This study investigates how anion and substrate binding cooperatively regulate reactivity in non-heme iron halogenases through electrostatic interactions in the active site.