Eileen W. Singleton

BMP signaling pathway member expression is enriched in enteric neural progenitors and required for zebrafish enteric nervous system development

This study identifies a time-dependent role for BMP signaling in zebrafish ENS development, showing BMP5 is required for proper enteric progenitor maintenance and GI tract colonization.

Expansion of a neural crest gene signature following ectopic MYCN expression in sympathoadrenal lineage cells in vivo

This study shows that MYCN overexpression in zebrafish sympathoadrenal progenitors leads to an expanded NCC gene signature and disrupted BMP signaling, contributing to neuroblastoma-like developmental changes.

A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system

This study uses a CRISPR-Cas9 screen in zebrafish to identify genes involved in enteric nervous system development, revealing opioid receptor involvement in ENS establishment.

In Toto imaging of early Enteric Nervous System Development reveals that gut colonization is tied to proliferation downstream of Ret

The paper uses in toto imaging and single-cell tracing in zebrafish to show that Ret signaling regulates ENCC proliferation and migration, leading to HSCR-like phenotypes.

Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafish

This study shows that elevated Hoxb5b in zebrafish expands vagal neural crest cells but impairs their ability to generate enteric neurons.

An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution

The paper creates a single-cell atlas of neural crest cell derivatives in zebrafish, identifying multiple cell types and subtypes and their gene expression patterns.