Thermostability improvement of Aspergillus awamori glucoamylase via directed evolution of its gene located on episomal expression vector in Pichia pastoris
Alexander Schmidt, Alexey V. Shvetsov, Elena Soboleva, Yury Kil, Vladimir Sergeev, M. A. Surzhik
This study improves the thermostability of Aspergillus awamori glucoamylase through directed evolution and identifies key mutations that enhance or reduce thermal stability.
Protein Engineering Design and Selection · Jun 1, 2019Peer-reviewedMolecular Biology