Enhanced Golden Gate Assembly: Evaluating Overhang Strength for Improved Ligation Efficiency
Patryk Strzelecki, Nicolas Joly, P. Hébraud, Elise Hoffmann, Grzegorz M. Cech, Anna Kłoska, Florent Busi, Wilfried Grange
The paper tests a hypothesis about overhang stability affecting ligation efficiency in Golden Gate Assembly and finds that strong overhangs improve efficiency.
Nucleic Acids Research · Sep 9, 2024Peer-reviewedMolecular Biology