Generating E. coli 0.5 controlled by a half-sized genome
Takahito Mukai, Aika Ohishi, Emi Hagiuda, Keita Shimamoto, Koki Yoshida, Masayuki Su’etsugu
The paper constructs a half-sized E. coli genome through genome-scale debugging and integrates it into a functional genome, resulting in a smaller genome than previously reported.
bioRxiv · Jun 3, 2026 · PreprintSynthetic Biology