PHYCUT: Scalable multiplex CRISPR/Cas9 editing forgenome engineering in the diatom Phaeodactylumtricornutum
Emily E. Stuckless, Gai Li, Samuel S. Slattery, Kira H. Dempsey, Tyler S. Browne, Gregory Brian Gloor, David R. Edgell
The paper develops PHYCUT, a CRISPR/Cas9 system for multiplex genome editing in the diatom Phaeodactylum tricornutum, demonstrating its use to reduce fucosylation of secreted proteins.
ACS Synthetic Biology · Apr 14, 2026Peer-reviewedSynthetic Biology