Nickel-NTA lipid-monolayer affinity grids allow for high-resolution structure determination by cryo-EM
Aleksandra Skrajna, Clara Lenger, Emily Robinson, Kevin S. Cannon, Reta D. Sarsam, Richard G. Ouellette, Alberta Mawulawoe Abotsi, Patrick Brennwald, Robert K. McGinty, Joshua D. Strauss, Richard W. Baker
This paper introduces a method using Ni-NTA lipid monolayers to improve cryo-EM grid preparation, enabling high-resolution structure determination of proteins and the nucleosome.
Journal of Structural Biology · Oct 11, 2025Peer-reviewedBiochemistry