Ultra-High Multiplexing Enables Near-Full-Length 16S rRNA Gene Amplicon Sequencing of Over 1,200 Gut Microbiome Samples on a Single Nanopore Flow Cell
Charlie H. McPhillips, Eoghan Thomas Reilly, Gladys Stolberg-Mathieu, Klara Nielsen, Adam Duun Gottlieb, Gjorgji Madjarov, Henrik Munch Roager, Dennis Sandris Nielsen, Łukasz Krych
This paper introduces a high-throughput sequencing method using Oxford Nanopore Technologies to sequence over 1,200 gut microbiome samples with near-full-length 16S rRNA gene amplicons, achieving high accuracy and scalability.
bioRxiv · Aug 29, 2026 · PreprintMicrobiology