A simple and efficient CRISPR technique for protein tagging
F. Zeng, V. Beck, S. Schuierer, I. Garnier, C. Manneville, C. Agarinis, L. Morelli, L. Quinn, J. Knehr, G. Roma, F. Bassilana, M. S. Nash
The paper introduces HiTag, a CRISPR-based method for protein tagging that works in up to 66% of transfected cells with one electroporation.
bioRxiv · Apr 5, 2019Cell Biology