F. Ijaz

Excessive F-Actin and Microtubule Formation Mediates Primary Cilia Shortening and Loss in Response to Increased Extracellular Osmotic Pressure

This study shows that hyperosmotic shock causes reversible primary cilia shortening and loss in mouse cells, mediated by excessive microtubule and F-actin formation.

Circadian oscillation in primary cilium length by clock genes regulate fibroblast cell migration

This study shows that circadian rhythms regulate primary cilia length in fibroblasts, which in turn affects cell migration and wound healing.

A pair of primers facing at the double-strand break site enables to detect NHEJ-mediated indel mutations at a 1-bp resolution

The paper introduces DST-PCR with TBE-High-Resolution PAGE to detect NHEJ-mediated indel mutations at 1-bp resolution in CRISPR/Cas9-engineered cell lines.

Knock-in of labeled proteins into 5'UTR enables highly efficient generation of stable cell lines

This study develops a CRISPR/Cas9-based method to knock-in tagged proteins into the 5'UTR of endogenous genes, achieving high-efficiency stable cell lines with proper protein localization and long-term expression.