Extraction-free direct PCR from dried serum spots permits HBV genotyping and RAS identification by Sanger and minION sequencing
S. Astbury, M. M. D. C. N. Soares, E. Peprah, B. J. King, A. C. G. Jardim, J. F. Shimizu, P. J. Jalal, C. H. Saeed, F. T. Sabeer, W. L. Irving, A. W. Tarr, C. P. McClure
This paper demonstrates workflows for HBV genotyping and RAS identification from dried serum spots using Sanger and MinION sequencing without DNA extraction.
bioRxiv · Feb 16, 2019Microbiology