High-throughput discovery of a dearomative cycloaddition enables dual photochemical-photophysical perturbative probing of protein function
Samuel D. Griggs, Yibo Zeng, Stefan Lohmann, Chris Arter, Samuel Liver, Yana Demyanenko, Sivaramakrishnan Ramadurai, Georgina L. Bonney, Maria Lopatniuk, Alasdair M. Mackenzie, Stanley W. Botchway, Andrew G. Leach, Graham John Hutchings, Shabaz Mohammed, Stephen P. Marsden, , Benjamin G. Davis
This paper develops a high-throughput method to discover a novel photochemical reaction for editing protein side chains and using it to probe protein function through combined photochemical and photophysical approaches.
bioRxiv · Oct 23, 2025 · PreprintBiochemistry