Ultra-low input single tube linked-read library method enables short-read NGS systems to generate highly accurate and economical long-range sequencing information for de novo genome assembly and haplotype phasing
Zhoutao Chen, Long Pham, Tsai-Chin Wu, Guoya Mo, Yu Xia, Peter L. Chang, Devin K. Porter, Tấn Đức Phan, Huu Che, Hao Tran, Vikas Bansal, Justin P. Shaffer, Pedro Belda‐Ferre, Greg C. Humphrey, Rob D. Knight, , Ming Lei
The paper introduces a low-cost, high-accuracy method for generating long-range sequencing data using short-read NGS systems, enabling de novo genome assembly and haplotype phasing with minimal DNA input.
Genome Research · Jun 1, 2020Peer-reviewedGenomics