Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-Like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system
C. De Marco Verissimo, J. Lopez-Corrales, A. L. Dorey, K. Cwiklinski, R. Lalor, N. E. D. Calvani, H. L. Jewhurst, S. Doyle, J. P. Dalton
This paper describes the production of a functionally active SARS-CoV-2 3CL-pro and a soluble 3CLpro-RBD chimeric protein in E. coli, showing improved RBD antigenicity and potential for vaccine development.
bioRxiv · Mar 25, 2022Biochemistry