Basis for discrimination by engineered CRISPR/Cas9 enzymes
M.-S. Liu, S. Gong, H.-H. Yu, K. Jung, K. A. Johnson, D. W. Taylor
This study investigates how engineered CRISPR/Cas9 variants improve off-target discrimination by analyzing their kinetic behavior during DNA cleavage.
bioRxiv · May 8, 2019 · PreprintBiochemistry