Labeling proteins within Drosophila embryos by combining FRET reporters, position-specific genomic integration, and GAL4-reponsive expression
Tzyy-Chyn Deng, Chia-Jung Hsieh, Michael De Freitas, Maria E. Boulina, Nima Sharifai, Hasitha Samarajeewa, Tatsumi Yanaba, James D. Baker, Michael D. Kim, Susan B. Zusman, Kenneth H. Wan, Charles Y. Yu, S Celniker, Akira Chiba
The paper introduces a method to label proteins in Drosophila embryos using FRET reporters, genomic integration, and GAL4-responsive expression to study protein interactions in situ.
bioRxiv · Aug 22, 2019 · PreprintDevelopmental Biology