Single cell variation and rapid emergence of phenotypic heterogeneity in cell lines- a cautionary tale for devotees of CRISPR-Cas9.
Hayley Bradley, John N. Barr, Martin A. Stacey
This study shows that CRISPR-Cas9-generated cell lines exhibit significant phenotypic heterogeneity, including variable susceptibility to viral infections, highlighting potential experimental artifacts in gene editing studies.
bioRxiv · Sep 27, 2024 · PreprintMicrobiology